FastQCFastQC Report
Sun 19 Mar 2023
SRR6026870_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6026870_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences202900941
Sequences flagged as poor quality0
Sequence length150
%GC51

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATAC2051490.10110795888324639No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTTACCG52653600.0142.65861
TTACCGT41140400.0142.636252
TACCGTC28835400.0142.189623
TTACCGG12325550.0140.17452
ACCGTCC23563400.0138.062274
AGTGGTC13567400.0137.949741
TACCGGC9124250.0137.588673
TACCGTG11470150.0136.928253
GTGGTCA12189650.0136.83712
ACCGTCA7300850.0136.433354
AGTTAGC2715400.0129.592912
ACCGTGC8073700.0129.32924
CTCGGTA3809950.0129.277682
GTTAACG2110100.0128.426911
ACCGTGA4358950.0128.419724
TGGTCAG4547300.0128.073653
AATCCGT2448150.0127.91021
TGGTCAT3837050.0127.2549743
GATAGAC2839450.0127.079311
TGGTCAA3475950.0126.668013
Produced by FastQC (version 0.11.5)