FastQCFastQC Report
Sun 19 Mar 2023
SRR6026869_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6026869_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences182352349
Sequences flagged as poor quality0
Sequence length150
%GC49

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATAC12782760.7009923409322246No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCCGCGTTGATAC4970230.27256188512274115No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT3613170.19814222409605484No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCCATGGACTCTGCGTTGATAC2623390.14386378976670053No Hit
CCATGTACTCTGCGTTGATACCACTGCTTCCCATGGACTCTGCGTTGATA2558000.1402778748959247No Hit
ACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACT2043440.11205997680896339No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCACGGACTCTGCGTTGAT1993960.10934654864248555No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGGACTCTGCGTTGAT1956010.10726541285190684No Hit
CCATGTACTCTGCGTTGATACCACTGCTTCCCACGGACTCTGCGTTGATA1859130.10195262140549667No Hit
CCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATA1849410.10141958741644727No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATAGAC21001400.0141.351091
ATGCCTA8640650.0139.640241
ATAGACA19048750.0139.551592
TGCCTAA7795800.0137.491762
TAGACAT6932950.0135.968523
GATCTGG17981000.0135.603591
GCCTAAT3253000.0132.820923
ATCTGGG14744850.0132.341032
TAGACAG7073600.0130.01763
TAGACAA6009250.0128.602523
ATAGACG2459750.0127.984642
GCCTAAG2500100.0126.457433
ATCTGGT4333850.0122.815472
GTCGTAG1900950.0122.662021
TCTGGGC6751950.0122.653213
AGTGGTC3542500.0121.393131
ATAGCGA1462700.0120.122361
CGCATAC4055950.0118.697881
AGCTGAA2354700.0117.5569842
TCGTAGA1593550.0117.294712
Produced by FastQC (version 0.11.5)