FastQCFastQC Report
Sun 19 Mar 2023
SRR6026869_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6026869_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences182352349
Sequences flagged as poor quality0
Sequence length150
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAG4355079023.882768847688386No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGA43817362.4028952870796307No Hit
AGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGA10399120.5702761745065318No Hit
AAGCAGTGGTATCAACGCAGAGTACATTGGAAGCAGTGGTATCAACGCAG6228300.3415530446498389No Hit
AGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTA5830420.31973374798698095No Hit
AGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTG4836310.26521786127361596No Hit
AAGCAGTGGTATAAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAG4128160.2263837029047539No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATAAACGCAG3676890.20163655802426764No Hit
CAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGT3654860.2004284573268645No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTTGTATCAACGCAG3640960.19966619678696873No Hit
CAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGT3629630.19904487218862202No Hit
AAGCAGTTGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAG3477230.19068742569364983No Hit
GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACAT3399790.18644070222533848No Hit
ATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGG3365640.18456795420825647No Hit
ATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAA3304810.18123210466567669No Hit
AACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAG3192000.17504572973721333No Hit
CGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCA3078310.16881109658751914No Hit
CAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAA3047340.1671127362335212No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAT2986450.16377359635767566No Hit
GTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTAC2803700.1537517896191181No Hit
AAGCAGTGGTATCAACGCATAGTACATGGGAAGCAGTGGTATCAACGCAG2742100.15037371413296133No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG2712430.14874664433305435No Hit
AAGCAGTGGTATCAACGCAGAGTACATGTGAAGCAGTGGTATCAACGCAG2654300.14555885978743274No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGTATCAACGCAGAGTACATGG2644730.14503405163154767No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAGGCATTGAGGCAGCCAGCG2582250.1416077179241601No Hit
GGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGC2574340.14117394232196043No Hit
CATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCA2524910.1384632560998707No Hit
AGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGT2431010.13331388453899215No Hit
ACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATC2373330.13015077749286355No Hit
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG2349610.12884999907514216No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAAGAGCACACGTCTGAACTC2329100.1277252534871377Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
AAGCAGTGGTATCAACGCAGAGTACATGGTAAGCAGTGGTATCAACGCAG2287560.12544724608949237No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA2283330.1252152775942579No Hit
GGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACG2247340.12324162602369328No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGTAAGCAGTGGTATCAACGCA2076370.11386582138297544No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGAAAGCAGTGGTATCAACGCAG2042770.11202323475416266No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGTGGTATCAACGCAGAGTACA1980820.10862596565728912No Hit
TGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAAC1945840.1067077013633644No Hit
GCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAG1934000.10605840893225896No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCAGT342091150.046.4821741
AGTGGTA345412600.045.8551835
AGCAGTG345134900.045.8101232
CAGTGGT346698900.045.6373334
GTATCAA349577500.045.326349
GCAGTGG348950200.045.3229373
GTGGTAT350595100.045.1996236
TGGTATC348041850.045.137047
GGTATCA349033150.045.0187848
GGTATAA3878150.038.9823388
TGGTATA3882200.038.965777
GTTGTAT3772600.037.0875936
TTGTATC3795650.036.7846347
GCAGTTG3863300.036.11583
CAGTTGT3914350.035.7188844
GTATAAA4264750.035.485719
TGTATCA4000350.034.925738
AGTTGTA4043450.034.610415
AGCAGTT4181200.033.4060632
ATGGGGC3155350.019.88899625-29
Produced by FastQC (version 0.11.5)