FastQCFastQC Report
Sun 19 Mar 2023
SRR6026867_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6026867_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences231026895
Sequences flagged as poor quality0
Sequence length150
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAG52193452.2591936752645188No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGCCGGGCGCGGTGGCTCACG9876950.42752381708631804No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGCCGGGCGCGGTGGCTCAC7916610.34267049297442187No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGA7836040.33918302022801283No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAGGCATTGAGGCAGCCAGCG5076810.21974973952707974No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA2624370.11359586510479656No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGATAAACTTCGCCTTAATTTT2462070.10657070900771098No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGAGGCATTGAGGCAGCCAGC2412170.10441078732413385No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGAAAAAAAAAAAAAAAAAAA2351670.10179204460156034No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCAGT175878550.0125.602771
TGGTATC179541500.0123.160197
AGTGGTA179447650.0123.102915
GTGGTAT179790350.0122.997056
GGTATCA179937450.0122.924938
AGCAGTG179822750.0122.8249362
GTATCAA180306000.0122.746099
CAGTGGT180206400.0122.583044
GCAGTGG182600400.0120.955343
AAGCGGT718650.033.8638651
ATGGGAT23462350.026.01730325-29
CATGGGG36711850.025.96505425-29
CATGGGT20245450.025.5225-29
ATGGGAC12995300.025.41286525-29
CATGGGC23741000.025.35903225-29
ATGGGAG25742650.025.29552825-29
ACATGGG173275350.025.26509520-24
AGAGTAC179086000.025.20066315-19
CAACGCA179817950.025.1567910-14
TACATGG177942800.025.1513420-24
Produced by FastQC (version 0.11.5)