FastQCFastQC Report
Sun 19 Mar 2023
SRR6026866_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6026866_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences224472747
Sequences flagged as poor quality0
Sequence length150
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATAC2438710.10864169626792156No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCTAATC5227100.0133.677721
AGTTAGC4293650.0131.879942
AGAGTAG20563200.0131.587721
CTAATCC4660550.0130.45092
AGTAGGC5169950.0129.583193
CAGATTC5295600.0128.985092
GTCGTAG3921050.0128.739011
ATAGCGA3213150.0128.656771
CTCTATC5621600.0128.52562
ACGTATC2458900.0128.282151
TATCAGC3582500.0128.183911
AGTAGTC3435800.0127.458483
TAGCGAC2896200.0127.227742
TCGTAGA3423300.0127.132772
CCTCTAT6357150.0126.739791
GAGTAGG13428250.0126.518282
TCTATCG2139700.0124.298313
CGAACTT2525600.0123.913181
TAGGGAC4364450.0123.603225
GAGTAGT9103650.0123.0894852
Produced by FastQC (version 0.11.5)