FastQCFastQC Report
Sun 19 Mar 2023
SRR6026866_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6026866_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences224472747
Sequences flagged as poor quality0
Sequence length150
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAG2971817713.239102473317175No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGA45050632.0069532093354745No Hit
AGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGA6973560.3106639934334657No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGTATCAACGCAGAGTACATGG6549730.29178285950231636No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGTGGTATCAACGCAGAGTACA4620990.20585973405493185No Hit
AGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTA4092220.182303645083472No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGTAAGCAGTGGTATCAACGCA4018020.17899812131759585No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAAGAGCACACGTCTGAACTC3829900.1706175939478301Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
AAGCAGTGGTATCAACGCAGAGTACATGGGGTATCAACGCAGAGTACATG3550470.15816931219717287No Hit
AGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTG3309040.14741388628348723No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAGGCATTGAGGCAGCCAGCG3200610.1425834557992022No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGTACATGGGAAGCAGTGGTAT2717090.121043201738873No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGCAGAGTACATGGGAAGCAGT2625820.11697722931149411No Hit
ATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGG2358930.1050875899870375No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGTACATGGGAAGCAGTGGTA2343750.10441133862900516No Hit
ATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAA2313850.10307932837833539No Hit
GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACAT2304470.1026614602796303No Hit
CAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGT2295840.10227700380928646No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCAGT284847150.060.2987021
AGTGGTA289137800.059.3956835
AGCAGTG290067100.059.2204782
CAGTGGT290650900.059.0528264
GTATCAA293809150.058.5796789
TGGTATC293606750.058.3999567
GCAGTGG294170200.058.3320053
GTGGTAT294426050.058.3155366
GGTATCA295272950.058.143418
GTTGTAT1997400.022.5313386
TTGTATC2044700.022.1447
ATGGGGC5741200.021.8205325-29
ATGGGCG2416800.021.71217325-29
ATGGGGG6978350.021.57041725-29
CATGGGG27548000.021.51075625-29
GCAGTTG2090600.021.3866623
ATGGGCT5033100.020.61838325-29
CATGGGC18984800.020.37597325-29
CAGTTGT2218300.020.3198974
ATGGGCC3432350.020.03671325-29
Produced by FastQC (version 0.11.5)