FastQCFastQC Report
Sun 19 Mar 2023
SRR6026864_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6026864_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences186862054
Sequences flagged as poor quality0
Sequence length150
%GC48

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATAC3888230.20808023441720278No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCCGCGTTGATAC2307920.1235092920470627No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTACGT20511750.0139.187012
CTCTACG23782000.0138.871
TACGTCC10841500.0138.832154
CTACGTC14314500.0138.384143
TCTACGG5630200.0134.795152
CTCGGTA5951350.0134.717642
CGAGTAA6849950.0134.312152
AATCCGT5018350.0133.753191
TACGTCA4149400.0133.149064
ATCCGTC4783100.0132.955862
TACGTGC3800000.0132.695114
GCGAGTA7716200.0132.621441
CTACGTG5942000.0131.832233
AGTCACT3992800.0130.310911
AGTTAGC3386350.0129.971342
TACGCAA4323350.0129.719042
ACGTCCC12008650.0129.432575
GTCACTA3614850.0127.1964652
GTACGCA4922550.0126.205691
ACGTGCC4091150.0126.053935
Produced by FastQC (version 0.11.5)