FastQCFastQC Report
Sun 19 Mar 2023
SRR6026863_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6026863_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences163952291
Sequences flagged as poor quality0
Sequence length150
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCAATG19659400.0141.832351
TCAATGA19638100.0140.55322
ACTAGTA10432300.0140.07992
ATAGCGA3426050.0139.026351
CATACCA13905950.0138.730071
GACTAGT10587100.0138.47361
TAGCGAC3435800.0138.384022
ATACCAA13909950.0137.6082
TATCAGC3811550.0137.048081
ATCAGCA3818800.0135.430442
AGAGTCA3466600.0135.20811
CTAGTAG3715750.0135.144683
GATCGCA2802550.0135.092532
GAGTCAA3456100.0134.603782
GAATCTG4259550.0134.422641
TACCAAG4875750.0134.195723
AATCTGA4273500.0133.804052
CTAGTAT3820100.0133.5493
CAATGAT7510800.0133.05673
CTAGTAA3291400.0132.590933
Produced by FastQC (version 0.11.5)