FastQCFastQC Report
Sun 19 Mar 2023
SRR6026862_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6026862_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences200247135
Sequences flagged as poor quality0
Sequence length150
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATA2075350.10363943534073534No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACGCA4321550.0138.155641
TACGCAA4322500.0137.76942
GCGAGTA5371250.0136.9651
ATGCCTA3155500.0136.787061
CTCGGTA5339700.0136.765692
CGAGTAA5353150.0136.679142
GGAGAAC14038100.0135.758881
CATCAAG5047300.0135.527981
GAGAACA14028900.0135.013372
ACTTCGA2307450.0134.373952
ATCATTC5995700.0134.141621
TCGTAGA3147600.0134.13652
GCTGATC3603100.0133.697592
ATCAAGT5148550.0132.850022
GATAGAC2453300.0132.570711
GCCACAT4960000.0132.31781
TGCCTAA3253750.0132.169862
GTCGTAG3219500.0131.949681
ACTAGTA2689450.0131.081732
ACGCTTA1609050.0130.712052
Produced by FastQC (version 0.11.5)