FastQCFastQC Report
Sun 19 Mar 2023
SRR6026862_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6026862_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences200247135
Sequences flagged as poor quality0
Sequence length150
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAG36196261.807579419301055No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAGGCATTGAGGCAGCCAGCG21609991.0791660015510334No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGACACTCGCTTCTGGAACGTC12857790.6420960779289052No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGAGGCATTGAGGCAGCCAGC10982830.5484637770223278No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGAATTCTGGACATTAATTA9657070.48225758635697835No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGACTCTTCTGGTCCCCACAGA9628210.4808163672354164No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGA8315130.41524339411897204No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGGAATTCTGGACATTAATT7255420.36232328617335774No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGACACACTCGCTTCTGGAACG5439280.2716283556316549No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGACTCTTCTGGTCCCCACAG3639300.18174042789675868No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGACACTCGCTTCTGGAACGT3592970.17942678680521448No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGGAATTCTCTTGCTTCAAC2492800.12448617554503338No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGATAAACTTCGCCTTAATTTT2138730.10680452431941162No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCAGT106771450.0120.28281
GGTATCA108586900.0118.1128548
GTGGTAT108703850.0118.07316
GTATCAA108791450.0117.974349
TGGTATC108966250.0117.644277
CAGTGGT109161650.0117.6200944
AGCAGTG109179650.0117.5869452
AGTGGTA109714750.0117.0283665
GCAGTGG112493000.0114.134393
CATGGGG30269000.026.18092225-29
ACACTCG2796600.025.75712630-34
ATGGGAC9492100.025.17603725-29
CGCAGAT4231950.025.0020690-94
ATGGGGG11508500.024.81767825-29
GAAACCG4284700.024.58277785-89
ACATGGG103709650.024.55190820-24
TACATGG107178500.024.26993820-24
AGAGTAC107830350.024.25769815-19
GTACATG107627400.024.22528520-24
AGTACAT107729400.024.21320-24
Produced by FastQC (version 0.11.5)