FastQCFastQC Report
Sun 19 Mar 2023
SRR6026861_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6026861_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences203514392
Sequences flagged as poor quality0
Sequence length150
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATAC3648550.17927724738012632No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AACATCG18823850.0139.055652
TATCAGC6207850.0135.288061
AAACATC19371500.0135.23071
CGAGTAA5980900.0134.951522
ACATCGT7589150.0134.838073
GCGAGTA6198800.0133.349381
ACATCGA5115600.0132.78293
ACATCGG5939650.0132.645553
ACGCTTA2399600.0131.887682
GTCTGTC5991450.0130.753141
ATCAGCA6286950.0129.813112
ATGCCTA2156050.0129.614181
GTCGTAG2572500.0127.406591
CATCGGT2401150.0126.817234
CATCGTT3599850.0126.564274
ACCTTAC3124550.0126.1441962
ATGCGTC2326700.0125.803273
ACGTGAT2727850.0125.761292
CATCGAT2110200.0125.577624
CATCGTA1851950.0124.8555154
Produced by FastQC (version 0.11.5)