FastQCFastQC Report
Sun 19 Mar 2023
SRR6026861_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6026861_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences203514392
Sequences flagged as poor quality0
Sequence length150
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAG2396970011.777889398603318No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGA73187253.5961707317485434No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAGGCATTGAGGCAGCCAGCG9717370.477478270922481No Hit
AGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGA5567360.27356099710137455No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGAGGCATTGAGGCAGCCAGC4963030.24386629128420556No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGCCGGGCGCGGTGGCTCACG4059610.19947532752376548No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGAATTCTGGACATTAATTA3635330.1786276618707143No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGCCGGGCGCGGTGGCTCAC3504460.17219715842012787No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGGAATTCTGGACATTAATT2682890.13182802324859658No Hit
AGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTA2680670.1317189400541265No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGAAAGCAGTGGTATCAACGCAG2475860.12165527831564855No Hit
AGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTG2118970.10411892639022796No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCAGT234215000.065.141881
AGTGGTA238200800.064.0168155
GTATCAA238489450.063.95179
AGCAGTG239646300.063.6421282
CAGTGGT239698650.063.625544
GGTATCA239650050.063.599978
TGGTATC240001800.063.4799967
GTGGTAT240065200.063.4701586
GCAGTGG243936550.062.5070843
CATGGGG24917500.023.1109625-29
TAGCGAG1077950.022.989012130-134
AAACCGC2267800.022.46934385-89
ATGGGGG9010250.022.43314225-29
GAAACCG2335650.022.02970185-89
ATGGGGC5287950.021.98925-29
ATGGGGT4837450.021.5316225-29
ATAGCGA1136450.021.265043130-134
CCCGTTC1150550.020.987078140-144
ATGGGAT13857450.020.43800225-29
CCGTTCT1214750.020.423782140-144
Produced by FastQC (version 0.11.5)