FastQCFastQC Report
Sun 19 Mar 2023
SRR6026860_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6026860_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences164774366
Sequences flagged as poor quality0
Sequence length150
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATAC6332510.3843140261270979No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT4632400.28113596261690366No Hit
ACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCACGTACT3818560.23174478486538372No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCCGCGTTGATAC3644810.22120006215044394No Hit
ACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACT3482300.21133748437545194No Hit
CCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATA3159510.19174766541052873No Hit
CCATGTACTCTGCGTTGATACCACTGCTTCCCATGGACTCTGCGTTGATA3021400.18336590049449802No Hit
AACAACCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGT2551620.15485539783536476No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCTCATG25466450.0142.331441
CTCATGG9710750.0139.23632
TCATGTG14035900.0138.063453
CTCATGT16585350.0138.043732
ATGTGCC7446550.0137.032625
ATGTGAC7501700.0136.837145
TGTGACT7335700.0134.972326
CATGTGA7638600.0134.037734
TGTGCCT7353450.0133.270896
ATGGGCC5143950.0131.701785
CATGTGC7766000.0130.871464
GTGACTC3033450.0130.555867
GTGACTG3975550.0130.382957
AACGTGA2618050.0129.782681
CGAACTT1940450.0129.642261
ATGGGAC4269050.0129.556825
CTGATCG1985850.0129.354253
GCTGATC5056900.0129.341432
ACGTGAT2565600.0129.185472
TATCAGC2804300.0128.889421
Produced by FastQC (version 0.11.5)