FastQCFastQC Report
Sun 19 Mar 2023
SRR6026860_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6026860_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences164774366
Sequences flagged as poor quality0
Sequence length150
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAG4011804624.3472616365582No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGA35494332.1541172247629827No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGAAAAAAAAAAAAAAAAAAA15210990.9231405569480388No Hit
AGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGA9965170.6047767163006411No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGAAAAAAAAAAAAAAAAAA5791300.35146850451240697No Hit
AGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTA5692000.34544208168884716No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA5589350.33921235054243815No Hit
AAGCAGTGGTATAAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAG5532550.3357652124117413No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAAAAAAAAAAAAAAAAAAAA5307460.322104713787823No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATAAACGCAG4739880.2876588218825251No Hit
AGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTG4691970.28475120942052357No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAAGAGCACACGTCTGAACTC3959420.24029344467330557Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGT3722970.22594351842324792No Hit
AAGCAGTGGTATCAACGCAGAGTACATTGGAAGCAGTGGTATCAACGCAG3714450.2254264477036434No Hit
CAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGT3633810.2205324825828794No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTTGTATCAACGCAG3450330.209397255395903No Hit
GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACAT3369200.20447355263985661No Hit
ATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGG3327380.20193553650207943No Hit
ATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAA3313530.20109499313746412No Hit
AAGCAGTTGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAG3274730.19874025793550923No Hit
AACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAG3196740.19400711880147667No Hit
CGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCA3074070.18656239284210022No Hit
CAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAA3034840.18418156134795868No Hit
GTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTAC2865390.17389780155488505No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAT2779340.16867550866498252No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG2738540.16619939535983405No Hit
GGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGC2636970.16003520838914956No Hit
AAGCAGTGGTATCAACGCATAGTACATGGGAAGCAGTGGTATCAACGCAG2572550.15612561968528527No Hit
CATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCA2534210.1537988014470649No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGTATCAACGCAGAGTACATGG2450350.1487094175801593No Hit
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG2413860.14649487408739295No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGTAAGCAGTGGTATCAACGCA2366690.14363217152357305No Hit
ACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATC2366260.1436060752313864No Hit
AGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGT2330270.14142187626441846No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGTAAGCAGTGGTATCAACGCAG2291760.13908474088742662No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA2253930.1367888740655206No Hit
GGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACG2236170.1357110365091619No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGTAGAATCTTAGTTCAACTTT2180640.1323409734739929No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGAAAGCAGTGGTATCAACGCAG2133140.12945824352314608No Hit
AAGCAGTGGTATCAACGCAGAGTACATGTGAAGCAGTGGTATCAACGCAG2123650.12888230442349266No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAGAGCACACGTCTGAACTCC2033060.12338448323934077Illumina Multiplexing Index Sequencing Primer (95% over 23bp)
GCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAG1977280.1199992479412726No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAGGCATTGAGGCAGCCAGCG1969770.11954347316378083No Hit
TGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAAC1963520.11916416659130098No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGCTAAACCTAGCCCCAAACC1910520.11594764685667186No Hit
TGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACA1799470.10920812767684994No Hit
GCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAG1792100.10876084936658169No Hit
GAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCA1715560.10411570935736447No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG1681840.1020692745375212No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGTGGTATCAACGCAGAGTACA1677780.1018228769880383No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCAGT310351250.047.1740881
AGTGGTA314360750.046.7040145
AGCAGTG313726000.046.5152
CAGTGGT315612450.046.4326934
GCAGTGG317163750.046.174743
GTATCAA316331150.046.162689
GTGGTAT319066100.046.020666
TGGTATC315356000.045.9665037
GGTATCA315404300.045.93928
GGTATAA4822650.041.5578468
TGGTATA4878900.041.1170587
GTATAAA5069750.039.4938359
TTGTATC3351300.037.653247
GTTGTAT3366500.037.367826
GCAGTTG3454500.036.3815233
CAGTTGT3457600.036.3322374
TGTATCA3494200.036.068068
AGTTGTA3660450.034.406385
AGCAGTT3669200.034.18852
AGTAGTG714600.020.5420682
Produced by FastQC (version 0.11.5)