FastQCFastQC Report
Sun 19 Mar 2023
SRR6026859_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6026859_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences154519082
Sequences flagged as poor quality0
Sequence length150
%GC48

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATAC2202960.14256879936679925No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTGTAGC16100800.0140.925961
TGTAGCC16201400.0140.079672
GTCACTA4706700.0139.849262
AGTCACT4759250.0138.6631
GCGAGTA4111200.0137.820771
ACGCTTA3470500.0137.729722
ACTTCGA2846700.0137.232132
GTAGCCA5203250.0136.777633
CGAGTAA4125450.0136.476352
GTAGCCT5583250.0135.685413
CAAGCTA3272850.0135.62372
GTAGCCG5886650.0135.400713
CAGATTC4337600.0134.86262
ACCGAGA5408950.0133.951312
ATAGCGA1673750.0133.238171
AACCGAG5552750.0133.193591
CATACCA4796650.0132.741881
TAGCCTA1666550.0132.720784
ACGTATC1410450.0132.576111
TAGCCGA1689250.0132.497244
Produced by FastQC (version 0.11.5)