FastQCFastQC Report
Sun 19 Mar 2023
SRR6026859_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6026859_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences154519082
Sequences flagged as poor quality0
Sequence length150
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAG26613471.722341969388609No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAGGCATTGAGGCAGCCAGCG10876940.703922121411516No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGA10669990.690528953569631No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGAGGCATTGAGGCAGCCAGC5047350.3266489765969487No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGATAAACTTCGCCTTAATTTT2210720.14307100271279116No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGAATTCTGGACATTAATTA1851900.1198492753147472No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCAGT104957150.0123.32151
GTATCAA107091150.0120.871389
GGTATCA106976150.0120.870018
TGGTATC106986250.0120.7994467
GTGGTAT107097150.0120.706826
AGTGGTA107288400.0120.532925
AGCAGTG107303400.0120.511692
CAGTGGT107660800.0120.127914
GCAGTGG109760050.0117.7945563
CATGGGG20930300.025.91274825-29
ATGGGAT16462000.025.59060125-29
ACATGGG100411650.025.40138620-24
ATGGGAG17349450.025.12825225-29
CATGGGC13112850.024.95116425-29
CATGGGT9051850.024.94011925-29
TACATGG105703450.024.81442620-24
ATGGGAC7605250.024.8000925-29
AGAGTAC106391700.024.79720515-19
GTACATG106076550.024.77531220-24
AGTACAT106217300.024.76216520-24
Produced by FastQC (version 0.11.5)