FastQCFastQC Report
Sun 19 Mar 2023
SRR6026858_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6026858_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences160929602
Sequences flagged as poor quality0
Sequence length150-151
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATGCCTA7176550.0141.584171
TGCCTAA6840750.0139.2232
ATCATTC8135250.0137.633641
GCCTAAT2951150.0134.423193
AGTGGTC6375350.0133.57681
TATCAGC2625000.0133.038681
TCATTCC7944250.0132.876592
GTGGTCA5947400.0132.568192
CATTCCG2670950.0132.453063
CCGACAA7395900.0131.274861
CGAACTT2306200.0130.758731
GCCTAAG2091050.0130.148353
AGTTAGC2078100.0129.745212
CGACAAC7256500.0127.963412
AGATCGC2615800.0127.2881851
ATCAGCA2536500.0126.791872
ACCTTAC2366900.0125.628372
ATAGCGA1561100.0124.154971
GATCGCA2485050.0123.5513152
AGCACCT2463800.0123.490011
Produced by FastQC (version 0.11.5)