FastQCFastQC Report
Sun 19 Mar 2023
SRR6026858_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6026858_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences160929602
Sequences flagged as poor quality0
Sequence length150-151
%GC44

[WARN]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAG2165693313.457395488991516No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGA52571673.2667495194575826No Hit
AGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGA3395690.21100468514176776No Hit
AGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTA3253200.2021505030503959No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAGGCATTGAGGCAGCCAGCG3247450.20179320396256245No Hit
AGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTG2617740.16266367203219703No Hit
CAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGT2271530.14115053860631557No Hit
CAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGT2146870.1334042943820864No Hit
GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACAT2122050.13186200510208185No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA2035980.12651370379950358No Hit
AACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAG1975330.12274497516000817No Hit
ATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGG1967800.12227706870237584No Hit
CGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCA1933200.12012706027819543No Hit
ATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAA1915660.11903714271287393No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAAGAGCACACGTCTGAACTC1915010.11899675238120579Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAA1859230.11553064053436235No Hit
GTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTAC1843300.1145407667136342No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGAGGCATTGAGGCAGCCAGC1739210.10807272113927181No Hit
GGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGC1688590.10492724638690153No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG1656890.10295744098093276No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGTATCAACGCAGAGTACATGG1653190.10272752678528341No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCAGT185995250.049.062541
AGCAGTG189144000.048.122932
AGTGGTA189233150.048.060635
GTATCAA190494650.047.8429649
CAGTGGT190264800.047.7922254
GTGGTAT190824750.047.658866
TGGTATC190883800.047.559457
GGTATCA191240900.047.5029958
GCAGTGG192212400.047.289883
CGTGGGA1649500.033.04089145
CACGGGA1117900.032.160065145
CGTGGGG511550.027.106117145
CATGCGA423750.026.173407145
CAGGGGA2049350.025.762014145
CACGGGC667350.024.560959145
CACGGGG456550.023.343256145
GGTATAA1093400.019.9485768
ATGGGGG3064650.018.71231825-29
CATGGGG11012400.018.1557525-29
CATGCGG268750.017.891497145
Produced by FastQC (version 0.11.5)