FastQCFastQC Report
Sun 19 Mar 2023
SRR6026857_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6026857_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences157101620
Sequences flagged as poor quality0
Sequence length150
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATA5073570.3229482929584049No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATAC4227980.2691238957306742No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT2003480.12752764739154185No Hit
CCATGTACTCTGCGTTGATACCACTGCTTCCCACGTACTCTGCGTTGATA1651730.10513768094816592No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATAGCGA10806150.0142.191971
TAGCGAC10826400.0141.768662
AGGCTAA8075400.0140.299711
GGCTAAC8110050.0139.610492
ACGCTTA4377450.0138.902662
ACTTCGA3981300.0138.585162
ACCTTAC7503050.0137.66232
TATCAGC3398150.0137.12451
ATGCCTA2563600.0137.012191
GCTAACT3055300.0136.958713
AGCGACT4059950.0136.33123
CACCTTA7606500.0135.608221
ATCAGCA3417250.0135.273012
GATAGAC2361300.0134.970781
CACTTCG4137400.0133.871431
ATCATTC4609150.0133.599691
GCCATGC4593600.0133.438222
CATTGGC4424450.0133.425922
AGCCATG4603750.0133.412381
CAGATTC3264700.0132.509962
Produced by FastQC (version 0.11.5)