FastQCFastQC Report
Sun 19 Mar 2023
SRR6026857_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6026857_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences157101620
Sequences flagged as poor quality0
Sequence length150
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAG73130964.655009922876671No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGA19014701.2103439799029443No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAGGCATTGAGGCAGCCAGCG5226530.33268466614157133No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGATAAACTTCGCCTTAATTTT2458650.1565006140611408No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGAGGCATTGAGGCAGCCAGC2330270.148328833273648No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA2099070.13361224410034728No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGAAAAAAAAAAAAAAAAAAA1757130.11184671424775887No Hit
AGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGA1609070.10242224109464945No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCAGT131902000.0104.5017551
GTATCAA134800450.0102.218269
AGTGGTA134739350.0102.156985
GGTATCA134938550.0101.929448
AGCAGTG135179150.0101.806522
TGGTATC135129150.0101.716797
GTGGTAT135322750.0101.659966
CAGTGGT135658800.0101.472794
GCAGTGG138080000.099.661873
ATGGGAT16793700.025.24682225-29
CATGGGG20424100.024.72902125-29
ATGGGAC8586000.024.57768225-29
ATGGGAG19050650.024.35389525-29
TTGTATC1111100.023.5536947
CATGGGC12295650.023.362325-29
CATGGGT9046700.023.33546325-29
ATGGGGG6871200.023.19667225-29
GTTGTAT1139900.022.9586016
ATGGGGT4308050.022.70761925-29
ATGGGCG1825100.022.32779125-29
Produced by FastQC (version 0.11.5)