FastQCFastQC Report
Sun 19 Mar 2023
SRR6026856_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6026856_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences234062546
Sequences flagged as poor quality0
Sequence length150
%GC42

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATAC6998370.2989957222801464No Hit
CCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATA2789580.11918096456149802No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGAACTT7949350.0137.31281
CTCGGTA5471450.0135.956512
CCGAAGT6897500.0135.933841
CGCATAC5333050.0135.321141
ACTTCGA3711900.0134.612562
CGAAGTA6447500.0133.68682
GTCACTA3864900.0133.002062
GAACTTA7587950.0132.485212
CACTTCG4286950.0131.256561
AGTCACT4260200.0131.210341
TCCTGTA5184850.0129.825272
GGCTAAC3798700.0129.353182
CCGTGAG4499850.0128.232181
GCTCGGT6561800.0128.010351
ACGCTTA1879650.0127.537212
ATCCTGT5838850.0127.11931
CGACTGG5059000.0126.508071
AGGCTAA4151000.0126.1698151
CAAGACT3905500.0125.926491
GCATACA5295250.0125.688132
Produced by FastQC (version 0.11.5)