FastQCFastQC Report
Sun 19 Mar 2023
SRR6026855_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6026855_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences134494961
Sequences flagged as poor quality0
Sequence length150-151
%GC54

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATAC1602770.11916952041050816No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGGCTAA6858250.0140.695681
GGCTAAC6564000.0140.050112
AGCCATG7014300.0138.30031
GCTAACT2334850.0136.37383
ATAGCGA2692300.0135.965061
GCCATGC6556050.0135.315862
TAGCGAC2530850.0133.041892
AGTCACT1913450.0131.806811
GTCACTA1817900.0129.880692
TCAAGTG5274850.0129.455021
TATCAGC1714100.0128.883761
AGTTAGC1255250.0128.235032
CAGATTC1904450.0127.592742
GCTAACA2167000.0124.436393
ATGCCTA946100.0124.237891
CCATGCT2345600.0122.309453
ATCAGCA1628700.0122.273752
CTAACGT844550.0122.266554
CTAACTG877050.0121.979914
GCTAACG2715400.0119.928593
Produced by FastQC (version 0.11.5)