FastQCFastQC Report
Sun 19 Mar 2023
SRR6026855_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6026855_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences134494961
Sequences flagged as poor quality0
Sequence length150-151
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAG3797656128.236419206813256No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGA74810705.562342220389952No Hit
AGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGA5513710.4099566228358548No Hit
AGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTA5200180.3866449688029576No Hit
AGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTG4297990.3195651322580033No Hit
CAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGT3614940.2687788429486217No Hit
CAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGT3456120.25697022210371134No Hit
GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACAT3426210.2547463469653707No Hit
AACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAG3230130.24016736210659967No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAAGAGCACACGTCTGAACTC3205840.2383613464894049Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCA3076610.22875280806988746No Hit
ATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGG3067920.22810668720889848No Hit
ATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAA3046130.2264865521616085No Hit
CAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAA2948300.21921267370009498No Hit
GTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTAC2899060.2155515699952506No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGAAAGCAGTGGTATCAACGCAG2773370.20620623846271832No Hit
GGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGC2713170.20173023433941142No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGTATCAACGCAGAGTACATGG2628010.1953983986061753No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG2625830.19523631074921832No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGTAAGCAGTGGTATCAACGCAG2457760.18273993179566034No Hit
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG2429250.18062014977646634No Hit
NAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAG2427510.1804907768998126No Hit
CATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCA2403770.17872565500799692No Hit
GGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACG2224440.16539206996758785No Hit
GCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAG2177430.16189677173109854No Hit
AGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGT2173280.1615882099850566No Hit
ACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATC2111790.15701629148767887No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA2108790.15679323480379315No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGTGGTATCAACGCAGAGTACA2087370.15520061008084907No Hit
AAGCAGTGGTATCAACGCAGAGTACATTGGAAGCAGTGGTATCAACGCAG2044520.15201461711268127No Hit
TGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAAC2007110.14923310026462627No Hit
GCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAG1928570.14339347628049798No Hit
TGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACA1921250.1428492179718168No Hit
GAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCA1904840.1416290979109619No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGTCAACGCAGAGTACATGGGA1887060.14030711529779916No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTTGTATCAACGCAG1877050.13956284949590045No Hit
AAGCAGTGGTATAAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAG1829430.13602219640035437No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAGCAGTGGTATCAACGCAGA1813900.1348675063001059No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAT1794030.13339012753050278No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG1766070.13131123923668783No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAGAGCACACGTCTGAACTCC1757210.13065247849694533Illumina Multiplexing Index Sequencing Primer (95% over 23bp)
AAGCAGTGGTATCAACGCAGAGTACATGGGGTATCAACGCAGAGTACATG1717410.12769325982406138No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGTACATGGGAAGCAGTGGTA1712900.12735793127595316No Hit
ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC1606410.11944016252028952No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGAGTACATGGGAAGCAGTGG1596740.11872117647589786No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATAAACGCAG1559170.11592776327136896No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCACCGCAG1444760.10742112487024699No Hit
TACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTAT1426850.1060894764674492No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGCAGAGTACATGGGAAGCAGT1398290.10396597683685711No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTGGGG519750.046.835835145
AAGCAGT286925150.038.911921
AGTGGTA289693800.038.445275
AGCAGTG290658750.038.2736852
GTATCAA292000350.038.1248669
CAGTGGT292229200.038.092784
GTGGTAT293117850.037.9991156
TGGTATC292755250.037.865177
GGTATCA293073200.037.8329858
GCAGTGG294477650.037.7737243
CGTGGGA2403600.034.3622145
CATGCGG266500.034.31389145
CACGGGG597600.031.750828145
GGTATAA1836800.031.710678
CAGGGGA2590250.031.671238145
CATGCGA957300.031.573486145
TGGTATA1896850.030.6991487
CACGGGA1622200.030.635073145
CACGGGC344550.029.075655145
GTATAAA2135350.027.3723149
Produced by FastQC (version 0.11.5)