FastQCFastQC Report
Sun 19 Mar 2023
SRR6026854_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6026854_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences165352859
Sequences flagged as poor quality0
Sequence length150
%GC48

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATA11940070.7220963745174797No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATAC10682890.6460662406810879No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT5209430.31504928499603385No Hit
CCATGTACTCTGCGTTGATACCACTGCTTCCCACGTACTCTGCGTTGATA3843940.23246891666989564No Hit
AGATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATA2535480.15333753618375598No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCACGTACTCTGCGTTGAT1908300.11540774145308247No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATAGCGA3023150.0138.025741
ACTAGTA4335100.0136.901252
CTCGGTA4673400.0136.618412
TAGCGAC3042700.0136.527632
ATGCCTA2157300.0135.826581
GACTAGT4379050.0135.754241
CTTCACA12202650.0135.677672
CTCAATG3548250.0134.108461
AGATCTG4503700.0134.06122
TCTTCAC12491400.0133.74421
TCGTAGA2417600.0133.060322
ACGTGAT2629350.0132.512312
GTCGTAG2455200.0132.314941
TCCTGTA4370650.0132.293762
AACGTGA2628900.0132.266831
TCAATGA3593700.0131.458542
ATCCTGT4417900.0131.192281
TGCCTAA2231350.0130.876022
CAGATCT4597650.0130.663451
ACGTATC1327650.0130.602051
Produced by FastQC (version 0.11.5)