FastQCFastQC Report
Sun 19 Mar 2023
SRR6026853_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6026853_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences157803568
Sequences flagged as poor quality0
Sequence length150
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCTAATC11958000.0139.7271
CTAATCC11931800.0139.686362
GATCGCA4361300.0138.423652
TAATCCG4291100.0138.392763
CATACCA9729050.0138.03511
CAGATTC6241500.0137.258182
ACGTATC2389050.0136.734421
ATACCAA9791950.0136.257062
ACGCTTA2324550.0135.75182
AGATCGC4468100.0135.29531
GTGCGAA4033100.0135.104282
ATGCCTA1733400.0134.27341
ACAGATT6383250.0133.864641
AGATTCG2183250.0133.184543
AGAGATC4437300.0132.808362
TTGGCTC15375350.0132.212312
ATCGCAT1656700.0132.03943
GATGTAC2016200.0131.947082
GCCATGC3579900.0131.635622
AGCCATG3574350.0131.449331
Produced by FastQC (version 0.11.5)