FastQCFastQC Report
Sun 19 Mar 2023
SRR6026853_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6026853_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences157803568
Sequences flagged as poor quality0
Sequence length150
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAG16054961.0174015837208445No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAGGCATTGAGGCAGCCAGCG13738720.870621632585646No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGACTCTTCTGGTCCCCACAGA6931020.43921820576325626No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGAGGCATTGAGGCAGCCAGC6636750.42057033843493324No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGACACTCGCTTCTGGAACGTC6454960.40905032007894776No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGA5853650.3709453515018114No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGAATTCTGGACATTAATTA5148360.3262511783003538No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGGAATTCTGGACATTAATT3783440.23975630259513522No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGCCGGGCGCGGTGGCTCACG3635960.2304105063074366No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGACACACTCGCTTCTGGAACG2883900.18275252179342358No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGCCGGGCGCGGTGGCTCAC2861320.1813216289253992No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGACTCTTCTGGTCCCCACAG2121160.13441774649860896No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCAGT76721350.0126.8135151
GGTATCA77711150.0125.06528
GTGGTAT77851750.0124.925716
TGGTATC77929150.0124.664997
GTATCAA78036250.0124.605459
CAGTGGT78206700.0124.39644
AGCAGTG78497850.0123.927262
AGTGGTA78553450.0123.8475345
GCAGTGG80326350.0121.110833
CATGGGG24082500.026.37894825-29
ACATGGG74648450.025.9107220-24
CAACGCA77568000.025.57542410-14
AGAGTAC77466700.025.5752115-19
TACATGG77112500.025.5742720-24
GTACATG77308650.025.55897920-24
AGTACAT77417150.025.52416220-24
ATCAACG77657450.025.49651710-14
CAGAGTA77742300.025.49547415-19
TCAACGC77955450.025.4200710-14
GCAGAGT77900150.025.41802815-19
Produced by FastQC (version 0.11.5)