FastQCFastQC Report
Sun 19 Mar 2023
SRR6026852_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6026852_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences158298575
Sequences flagged as poor quality0
Sequence length150-151
%GC50

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATAC3629960.22931097137166268No Hit
ACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACT2437290.15396790527015167No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATGCCTA3567600.0138.861711
TGCCTAA3395400.0136.41082
ACGCTTA3108750.0136.104952
CTCAATG2238450.0131.937791
GCCTAAT1478050.0127.713893
ATGAATC2668450.0126.906482
AACGCTT3612350.0125.157881
GCCACAT2808050.0124.632151
CAGATTC1652650.0124.43812
CGAACTT1187150.0124.404051
ATAGCGA1836500.0123.037721
CTGGCAT1392900.0122.526011
CGTGATG4643500.0122.468411
CAAGCTA1676800.0121.868542
ACGTATC1097500.0121.752151
TAGCGAC1710200.0121.033992
TCAATGA2197150.0120.536962
AGAGATC3463400.0120.24012
GCCTAAG1146500.0120.02893
CGCTTAA1119550.0119.433823
Produced by FastQC (version 0.11.5)