FastQCFastQC Report
Sun 19 Mar 2023
SRR6026852_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6026852_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences158298575
Sequences flagged as poor quality0
Sequence length150-151
%GC46

[WARN]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAG4075735125.747137016236564No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGA79249795.006348920070821No Hit
AGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGA7165290.4526439988483788No Hit
AGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTA7148370.45157513262516735No Hit
AGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTG5860380.3702105341125149No Hit
CAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGT5132740.3242442327734157No Hit
CAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGT4897190.3093641240927153No Hit
GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACAT4840580.3057879706118643No Hit
AACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAG4492460.28379661661515276No Hit
CGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCA4401680.2780618840062205No Hit
ATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGG4317950.27277251232362637No Hit
ATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAA4280850.2704288399311238No Hit
CAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAA4189210.2646397796063546No Hit
GTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTAC4062970.2566649762955857No Hit
GGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGC3927830.2481279442976666No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG3643840.23018779543656662No Hit
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG3446950.2177499070980266No Hit
CATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCA3445390.2176513591483688No Hit
GGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACG3143900.19860570444174877No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAAGAGCACACGTCTGAACTC3141770.19847114858740833Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAG3090610.1952392812127336No Hit
ACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATC3057780.1931653522465379No Hit
AGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGT3045400.1923832858255357No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA2971810.18773447581571723No Hit
TGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAAC2898440.18309956359367102No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGTATCAACGCAGAGTACATGG2753610.17395039721614677No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGAAAGCAGTGGTATCAACGCAG2708710.17111398507535522No Hit
TGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACA2649880.1673975902815297No Hit
GAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCA2647450.16724408289840892No Hit
GCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAG2604090.16450495527202313No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGAAAAAAAAAAAAAAAAAAA2590710.16365971708841978No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG2464170.15566596224887053No Hit
ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC2283700.14426535425224138No Hit
AAGCAGTGGTATCAACGCAGAGTACATTGGAAGCAGTGGTATCAACGCAG2259370.14272838526815543No Hit
NAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAG2248330.14203096900903878No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGTAAGCAGTGGTATCAACGCAG2197260.13880478709299815No Hit
TACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTAT2062550.13029491895299752No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGTGGTATCAACGCAGAGTACA2016870.1274092328373771No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGTCAACGCAGAGTACATGGGA1910520.12069091588474501No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGTATCAACGCAGAGTACATG1865540.11784945000294537No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1852960.11705474922942294No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAGCAGTGGTATCAACGCAGA1820190.11498461056898333No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTTGTATCAACGCAG1769480.11178117048747911No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAGGCATTGAGGCAGCCAGCG1748590.11046151236674114No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCACCGCAG1735490.1096339622766661No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAT1648330.10412791144835006No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAGAGCACACGTCTGAACTCC1642290.10374635400223911Illumina Multiplexing Index Sequencing Primer (95% over 23bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CATGCGA934050.038.664806145
AAGCAGT315173350.036.9733661
AGCAGTG317873250.036.5601272
AGTGGTA319037800.036.431725
CAGTGGT321092550.036.186914
GCAGTGG322062450.036.0621453
GTGGTAT323033950.035.9885946
GTATCAA324089500.035.8819929
TGGTATC323254550.035.8396537
GGTATCA323766350.035.7909248
CATAGGA2112550.032.155827145
CACGGGA1500100.027.502398145
CGTGGGA1668900.027.485912145
CATGCGG265600.026.783974145
CATCGGA750950.026.685324145
CAGGGGA1969450.026.499977145
CATGAGA2015850.025.757023145
CATGTGA4725500.023.34908145
GGTATAA1957650.022.9464388
CATGTGC1652900.022.359137145
Produced by FastQC (version 0.11.5)