FastQCFastQC Report
Sun 19 Mar 2023
SRR6026851_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6026851_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences190305676
Sequences flagged as poor quality0
Sequence length150
%GC47

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATGCCTA9001400.0141.591611
TATCAGC8727650.0140.539411
CTAACGA9506900.0140.303962
ATCAGCA8637050.0139.997822
TGCCTAA9046050.0139.867022
GTGCGAA10871950.0139.16012
GCTAACG9705700.0138.781
TCTGTCA15677150.0138.658082
CATCAAG5460550.0137.199841
TAACGAT3433750.0136.642913
GTACGCA3272100.0136.537611
AACGTGA4580250.0136.447161
ACGTGAT4600000.0136.325182
TGCGAAT3999300.0136.292983
ATCAAGT5499350.0136.285062
AACATCG7376950.0135.882312
CTGTCAG5414100.0135.86713
TACGCAA3255750.0135.858612
CCGTCTA7537350.0135.661622
TAACGAG3487600.0135.37953
Produced by FastQC (version 0.11.5)