FastQCFastQC Report
Sun 19 Mar 2023
SRR6026851_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6026851_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences190305676
Sequences flagged as poor quality0
Sequence length150
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAG20227471.0628936784838723No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGA5524370.29028929226472466No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA4819660.25325886759152677No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAGGCATTGAGGCAGCCAGCG3233130.16989141196187968No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGAAAAAAAAAAAAAAAAAAA1937470.10180831390441554No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCAGT105462100.0128.254731
GGTATCA107226250.0126.017088
GTGGTAT107242450.0125.9756
TGGTATC107222950.0125.964337
GTATCAA107612750.0125.687179
AGTGGTA107563950.0125.6460655
AGCAGTG107662150.0125.529012
CAGTGGT107856900.0125.314034
GCAGTGG110107150.0122.7089843
ACATGGG103461050.025.98120120-24
CAACGCA106965150.025.86000410-14
TACATGG106317150.025.7914520-24
ATGGGAT18384600.025.75654625-29
TCAACGC107300050.025.74560510-14
ATCAACG107206650.025.7252710-14
AGAGTAC107175650.025.72306315-19
GTACATG106853600.025.70830720-24
GCAGAGT107206300.025.69161415-19
CAGAGTA107474600.025.66193615-19
AGTACAT107087850.025.65955220-24
Produced by FastQC (version 0.11.5)