FastQCFastQC Report
Sun 19 Mar 2023
SRR6026850_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6026850_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences224998146
Sequences flagged as poor quality0
Sequence length150
%GC41

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AACATCG68983400.0141.451552
AAACATC69464100.0141.07461
ACATCGT26901000.0139.832723
ACATCGG21643500.0139.830693
CGCATAC8494150.0139.789021
ACATCGA17057150.0139.364613
CATCGGT8114750.0137.279884
CATCGAT6636550.0136.905964
CATCGTG8315150.0136.81494
CATCGTA5721800.0136.367524
ACATCGC5254850.0135.845343
CATCGTT11656000.0135.704064
CATCGAG5791300.0134.932534
GCATACA8665000.0134.867862
CATCGGA4566000.0134.645724
GCGAGTA4143700.0134.109131
CATCGGG7777200.0134.098044
ATCCGTC3693450.0133.378192
TATCAGC4492800.0133.006271
CATCGAA4124900.0132.104544
Produced by FastQC (version 0.11.5)