FastQCFastQC Report
Sun 19 Mar 2023
SRR6026850_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6026850_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences224998146
Sequences flagged as poor quality0
Sequence length150
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAG50889742.261784859329463No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGA15416740.6851940904437497No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA8058010.3581367288244233No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAGGCATTGAGGCAGCCAGCG6686960.29720067115575255No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGAGGCATTGAGGCAGCCAGC3123120.1388064770987046No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCAGT158801100.0119.402781
GGTATCA160095600.0117.513058
GTATCAA160739000.0117.494599
TGGTATC160397400.0117.346157
GTGGTAT161166000.0117.29236
AGTGGTA161278400.0117.276015
AGCAGTG161176850.0117.238752
CAGTGGT161645000.0116.9443054
GCAGTGG164799050.0114.667243
GGTATAA1769450.048.964028
TGGTATA1920550.045.1428537
TTGTATC2065200.037.3400547
GTTGTAT2086850.036.9699256
GCAGTTG2124150.036.2675133
TGTATCA2268400.034.017278
CAGTTGT2351350.032.8948944
GTATCGA484300.030.2372449
GTATAAA2969150.029.3665949
GGTATCG501100.029.1803728
AGCAGTT2841500.027.2612922
Produced by FastQC (version 0.11.5)