FastQCFastQC Report
Sun 19 Mar 2023
SRR6026849_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6026849_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences219289944
Sequences flagged as poor quality0
Sequence length150
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAG3196911714.578469225200768No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGA93085994.244881835530041No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAGGCATTGAGGCAGCCAGCG15037850.6857519193857791No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGAGGCATTGAGGCAGCCAGC7009820.31965989284032104No Hit
AGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGA5816940.2652625056076443No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGAATTCTGGACATTAATTA5159830.2352971552585193No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA4413840.20127872347853762No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGGAATTCTGGACATTAATT3522770.1606443932513385No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGAAAGCAGTGGTATCAACGCAG3077580.14034296073330205No Hit
AAGCAGTGGTATCAACGCAGAGCACACGTCTGAACTCCAGTCACGCATTC2530100.11537692763513131TruSeq Adapter, Index 5 (96% over 29bp)
AAGCAGTGGTATCAACGCAGAGTACATGGGACACTCGCTTCTGGAACGTC2513870.11463681161777305No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGTAAGCAGTGGTATCAACGCAG2340510.10673129635164666No Hit
AGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTA2266200.10334263207254046No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAAGAGCACACGTCTGAACTC2194220.10006021981564281Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCAGT293810200.067.055181
AGTGGTA298638050.065.807225
GTATCAA299112050.065.5887769
AGCAGTG299657400.065.528722
CAGTGGT301326450.065.181534
GGTATCA300182650.065.119778
GTGGTAT302505950.064.936276
TGGTATC301651550.064.844727
GCAGTGG306158400.064.130973
GGTATAA2254450.039.886498
TGGTATA2319450.038.8481837
TTGTATC2348750.031.1556427
GTTGTAT2389400.030.5954636
TGTATCA2417650.030.2138028
GCAGTTG2434550.030.049233
GTATAAA3113800.028.9387269
CAGTTGT2738100.026.7968434
AGCAGTT2839300.025.8647042
CATGGGG30119400.023.66141125-29
AAACCGC3278700.023.45757185-89
Produced by FastQC (version 0.11.5)