FastQCFastQC Report
Sun 19 Mar 2023
SRR6026847_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6026847_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences203357167
Sequences flagged as poor quality0
Sequence length150
%GC47

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCATGTACTCTGCGTTGATACCACTGCTTCCCATGGACTCTGCGTTGATA2867370.14100166924532342No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATAC2304730.11333409262138275No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGTATC35932100.0141.779451
GCTCATG25860100.0140.966541
TCATGTC15280400.0139.46363
CTCATGT23788000.0139.43342
CGTATCA34277150.0137.061942
GTATCAG11296500.0136.41533
CGAACTT5388100.0134.780761
GTATCAA9278650.0134.728733
ATCCGTC4844450.0133.959442
ATGTCCC11520450.0133.344075
CGTATCG2888850.0132.879842
ATGTCAC6082350.0131.088415
AATCCGT5385450.0130.541781
GAACTTA5109550.0130.223072
CATGTCA6032650.0129.50914
ATGTGCC5760650.0128.063265
ATGTGAC5108600.0127.633415
ATGCCTA2134200.0127.020051
TATCAGG3972950.0126.925324
GTATCAT12853700.0125.769843
Produced by FastQC (version 0.11.5)