FastQCFastQC Report
Sun 19 Mar 2023
SRR6026847_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6026847_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences203357167
Sequences flagged as poor quality0
Sequence length150
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAG3435854316.895663677297392No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGA64711643.1821666752468087No Hit
AGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGA8190100.40274459566994264No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGTATCAACGCAGAGTACATGG5569810.27389297766918635No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAGGCATTGAGGCAGCCAGCG5266630.2589842333907022No Hit
AGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTA4455580.21910120335222805No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGTGGTATCAACGCAGAGTACA4266380.2097973758652922No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAAGAGCACACGTCTGAACTC4064950.1998921434620497Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
AGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTG3611840.17761065682037164No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGTAAGCAGTGGTATCAACGCA3169030.15583566818670325No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGTATCAACGCAGAGTACATG2694770.13251413951886928No Hit
CAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGT2628660.12926320910046904No Hit
ATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGG2619470.12881129485837103No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGAGGCATTGAGGCAGCCAGC2616650.12867262258821693No Hit
CAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGT2577480.12674645492086345No Hit
GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACAT2555260.12565379611135122No Hit
ATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAA2513190.12358502220873287No Hit
CGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCA2393380.11769341770973825No Hit
AACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAG2389070.11748147533939632No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGTACATGGGAAGCAGTGGTAT2314250.1138022344695626No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGCAGAGTACATGGGAAGCAGT2305500.11337195703557378No Hit
CAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAA2256010.1109383078689329No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG2161070.10626967477374426No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGTAGCAGTGGTATCAACGCAG2071140.10184740624361668No Hit
GTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTAC2039780.10030529192020067No Hit
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG2039080.10027086972548156No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCAGT294188750.050.5333021
AGTGGTA299253400.049.601265
AGCAGTG299730900.049.529182
CAGTGGT300111200.049.4373474
GTATCAA302503950.049.146339
TGGTATC303032000.048.8848727
GCAGTGG304047650.048.7901343
GTGGTAT304192050.048.7830436
GGTATCA304366200.048.7229278
GTTGTAT1812050.021.7249186
TTGTATC1840700.021.3907557
ATGGGGG6137450.020.46544325-29
GCAGTTG1924800.020.4346053
ATGGGGC3947250.019.77269425-29
CATGGGG22403100.019.4122125-29
ATGGGCT3711400.019.02641725-29
TGTATCA2097050.018.9681368
ATGGGAT10359500.018.93400625-29
CAGTTGT2109900.018.8216574
ATGGGCG1563650.018.80206525-29
Produced by FastQC (version 0.11.5)