FastQCFastQC Report
Sun 19 Mar 2023
SRR6026845_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6026845_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences223327005
Sequences flagged as poor quality0
Sequence length150
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTGGCAT17460550.0139.578161
TGGCATA16630900.0138.95842
CTCGGTA5841800.0137.405362
ATGCCTA5902450.0137.09491
CAATGGA17674350.0136.846681
GAGCTGA9261000.0135.78071
AACATCG8107400.0135.556922
GCTGATC5924850.0134.058362
CGCTGAT5938800.0133.765371
AGCTGAA8736350.0133.615192
TGCCTAA5699500.0133.164732
AGATCGC4454900.0132.717321
GGCATAG5655450.0131.92143
GAATCTG8187950.0131.90541
GGCATAT5952650.0131.892333
GATCGCA4279200.0130.603062
AATCTGA7708550.0130.584172
ACTAGTA4928850.0130.193652
GCTCGGT6598900.0129.222021
ATCATTC7817900.0128.929461
Produced by FastQC (version 0.11.5)