FastQCFastQC Report
Sun 19 Mar 2023
SRR6026845_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6026845_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences223327005
Sequences flagged as poor quality0
Sequence length150
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAG17592200.7877327688158446No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGCCGGGCGCGGTGGCTCACG6265900.28057063676647614No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA5226370.23402319840361446No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGAAAAAAAAAAAAAAAAAAA4814750.2155919298698337No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGCCGGGCGCGGTGGCTCAC4742980.21237825671821461No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGA4612490.2065352553310783No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGATAAACTTCGCCTTAATTTT2841030.12721390321783968No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAGGCATTGAGGCAGCCAGCG2618760.11726123314106146No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCAGT162509500.0135.692931
TGGTATC165078600.0133.625027
GTGGTAT165334900.0133.406946
AGTGGTA165451400.0133.370795
GGTATCA165441050.0133.345448
GTATCAA165663550.0133.176459
AGCAGTG165740500.0133.131242
CAGTGGT165932000.0132.992584
GCAGTGG168497050.0130.96913
CAACGCA164895350.027.32385310-14
ATCAACG164950650.027.2559310-14
TCAACGC165111000.027.25348910-14
ACATGGG160403550.027.25177220-24
AACGCAG165651850.027.19412410-14
AGAGTAC165408600.027.18043515-19
CGCAGAG165418300.027.17986315-19
TACATGG164917250.027.11600520-24
CAGAGTA165913700.027.10704615-19
GCAGAGT165805950.027.10371215-19
AGTACAT165355100.027.09783720-24
Produced by FastQC (version 0.11.5)