FastQCFastQC Report
Sun 19 Mar 2023
SRR6026844_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6026844_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences182682393
Sequences flagged as poor quality0
Sequence length150
%GC35

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATAC3226960.17664318640713228No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCGTAG15365150.0142.163541
TCGTAGA13735900.0141.178342
ACGTATC4196250.0138.26211
GTTACCG6534200.0136.10921
ATGCCTA3516650.0135.8261
ACGTGAT4593650.0135.112672
ACTTCGA2743700.0134.877822
CGTAGAG4608400.0134.843283
AACGTGA4715250.0134.051251
GATAGAC3108200.0133.921361
ATCAGCA3659650.0133.538562
TATCAGC4073100.0133.105221
TACCGTC1652000.0132.409333
CACTTCG3083800.0132.077271
CTGGCAT3133050.0132.000811
TGCCTAA3336250.0131.83052
GCGAGTA2870350.0131.73631
CGTAGAT5528150.0131.708943
CGTAGAA4297850.0131.281883
GCCACAT3586450.0129.96411
Produced by FastQC (version 0.11.5)