FastQCFastQC Report
Sun 19 Mar 2023
SRR6026844_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6026844_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences182682393
Sequences flagged as poor quality0
Sequence length150
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAG117872736.452331177860146No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGAAAAAAAAAAAAAAAAAAA45731892.5033550989229707No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA18350271.0044903451642435No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGAAAAAAAAAAAAAAAAAA17857640.9775238711702227No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAAAAAAAAAAAAAAAAAAAA16083280.880395736878704No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGA13679360.7488056060224698No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGTAGAATCTTAGTTCAACTTT6724870.368118125100321No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGCTAAACCTAGCCCCAAACC4848750.26541966745530865No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGCCGGGCGCGGTGGCTCACG4521860.24752577003958995No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGATAGGTTTGGTCCTAGCCTT3620260.19817235479283438No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAGGCATTGAGGCAGCCAGCG3408590.1865855786112896No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAGAAAAAAAAAAAAAAAAAA3389100.1855186996592496No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGCCGGGCGCGGTGGCTCAC3285670.1798569608183313No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGAGAAAAAAAAAAAAAAAAA2974540.16282576285279993No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGATAAACTTCGCCTTAATTTT2830860.15496074654550862No Hit
AGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGA2716460.14869851195785463No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGAGAAAAAAAAAAAAAAAAAAA2552700.13973432020895413No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGCTAAACCTAGCCCCAAACCC2261420.12378970752808126No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGAAAAAAGAAAAAGAAAGAA2076680.11367707450602534No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGGAAAAAAAAAAAAAAAAA1879560.1028867626011446No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGTGCCCCTCATTTACATAAAT1856820.10164197925740988No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCAGT141061800.082.619911
AGTGGTA143391900.081.1414265
AGCAGTG143340700.080.925022
CAGTGGT144137150.080.5910344
GTATCAA144426600.080.527719
GGTATCA144246250.080.171738
GTGGTAT145427400.080.003496
TGGTATC144707450.079.9429867
GCAGTGG145406950.079.778393
GGTATAA1484450.053.4872938
TGGTATA1538600.051.5486877
TTGTATC1648700.046.6433457
GTTGTAT1661150.046.319776
TGTATCA1763500.043.5824748
CAGTTGT1823150.042.2625584
GCAGTTG1880100.040.959713
GTATAAA1972900.040.288729
AGCAGTT2118100.036.6092762
AGTTGTA2572250.030.2266335
CATGGGG27937800.025.22953225-29
Produced by FastQC (version 0.11.5)