FastQCFastQC Report
Sun 19 Mar 2023
SRR6026843_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6026843_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences184691864
Sequences flagged as poor quality0
Sequence length150
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAG39681512.1485250698428167No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAGGCATTGAGGCAGCCAGCG4291940.23238381523941953No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGA4057180.21967291423297344No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGAGGCATTGAGGCAGCCAGC2061710.11162971423581496No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCAGT99316250.0111.615721
TGGTATC99196300.0110.2907267
GTGGTAT100359450.0110.098176
GGTATCA99431850.0110.0528268
GTATCAA100309000.0109.984739
AGTGGTA100681150.0109.746895
CAGTGGT100533250.0109.6815264
AGCAGTG100353550.0109.610572
GCAGTGG103372700.0106.6038363
GGTATAA1824750.063.679298
TGGTATA1947750.059.6801267
GTTGTAT1814350.053.3867846
TTGTATC1885550.051.286897
GCAGTTG2010800.048.0053563
CAGTTGT2099250.046.135474
TGTATCA2193450.044.1269878
GTATAAA2742750.042.528479
AGTTGTA2443100.039.8858875
AGCAGTT2716550.035.711382
CATGGGG18617600.025.48247725-29
Produced by FastQC (version 0.11.5)