FastQCFastQC Report
Sun 19 Mar 2023
SRR6026842_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6026842_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences146068947
Sequences flagged as poor quality0
Sequence length150-151
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATAC1562680.10698235539412769No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATAGCGA3302250.0138.806171
TAGCGAC3093900.0137.754212
AGTTAGC3453800.0137.127982
GGCTAAC3925850.0136.261982
GATAGAC2743000.0134.918951
AGGCTAA4191100.0134.843721
ATCCGTC2877000.0134.776342
CCTAATC3171450.0133.367781
TACGCAA2052350.0131.627272
CTAATCC3011100.0130.965822
CTCAATG2532100.0130.60731
GGCTTCA4031350.0130.34552
ATGAATC3281500.0129.795062
CAAGCTA2125950.0129.687412
GTACGCA2287100.0129.666321
GAGTTAG4026450.0129.21951
ACCTTAC2461600.0129.173322
AGCACCT2726750.0128.942721
GCTAACT1479100.0127.61983
AACTCAC3158200.0127.026571
Produced by FastQC (version 0.11.5)