FastQCFastQC Report
Sun 19 Mar 2023
SRR6026842_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6026842_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences146068947
Sequences flagged as poor quality0
Sequence length150-151
%GC44

[WARN]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAG1709086411.700545770347752No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGA33817752.315190921448896No Hit
AGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTA3007480.20589454923639586No Hit
AGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGA2936790.20105505381646926No Hit
AGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTG2502600.17133005004821455No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAGGCATTGAGGCAGCCAGCG2237230.15316260204162355No Hit
CAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGT1935350.13249564946887718No Hit
CAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGT1911380.1308546435951236No Hit
GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACAT1867060.12782046001878825No Hit
ATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGG1819790.1245843170211941No Hit
ATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAA1807860.12376757942945943No Hit
AACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAG1785540.12223953390996924No Hit
CGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCA1714230.11735759278116793No Hit
CAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAA1668680.11423920239529077No Hit
GTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTAC1561890.10692827134572279No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG1483770.10158011202750711No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCAGT143210100.045.270511
AGTGGTA144952400.044.5971375
AGCAGTG145084500.044.5681722
GTATCAA145355300.044.547719
CAGTGGT145895000.044.2902954
GTGGTAT146252200.044.2004246
GGTATCA146053250.044.1498378
TGGTATC146269250.044.078977
GCAGTGG147852150.043.6774523
CACGGGA1228850.031.570223145
CGTGGGA1330650.031.171227145
CACGGGG406000.028.062763145
CATGCGA383100.025.071432145
CAGGGGA1838250.023.551441145
CATCGGA536650.023.397152145
CGTGGGG437100.021.298887145
GGTATAA1024950.021.0989768
CACGGGC303250.020.407616145
TGGTATA1134600.019.142877
GTTGTAT1500750.018.9637016
Produced by FastQC (version 0.11.5)