FastQCFastQC Report
Sun 19 Mar 2023
SRR6026841_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6026841_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences184156353
Sequences flagged as poor quality0
Sequence length150
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAG40571282.2030888068249266No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGA12960830.7037948889007375No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAGGCATTGAGGCAGCCAGCG11116190.6036278314004188No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGAATTCTGGACATTAATTA6062500.3292039563793925No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGAGGCATTGAGGCAGCCAGC5527630.3001596149115746No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGGAATTCTGGACATTAATT4577080.2485431496354622No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGACACTCGCTTCTGGAACGTC4347130.23605647750854405No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGACTCTTCTGGTCCCCACAGA4334370.23536358802674595No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGACACACTCGCTTCTGGAACG2042110.11089001094629627No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1871530.1016272297703463No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCAGT118036150.0118.5852051
GGTATCA120199050.0116.258018
GTATCAA120258400.0116.249519
GTGGTAT120323050.0116.113966
TGGTATC120451050.0116.004377
AGTGGTA120545050.0115.946835
AGCAGTG120803250.0115.842052
CAGTGGT120942400.0115.700864
GCAGTGG124583050.0112.297263
CATGGGG31060600.026.26970725-29
ATGGGGG11680900.025.20813625-29
ATGGGAC7757800.024.61284425-29
CATGGGC15207000.024.53092825-29
ACATGGG112419800.024.44626420-24
TACATGG118464100.023.94909720-24
AGTACAT118768250.023.92811620-24
AGAGTAC119212650.023.91964315-19
GTACATG118823100.023.9059720-24
ATGGGGT6330250.023.88628425-29
CAGAGTA119508900.023.86297415-19
Produced by FastQC (version 0.11.5)