FastQCFastQC Report
Sun 19 Mar 2023
SRR6026840_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6026840_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences180235456
Sequences flagged as poor quality0
Sequence length150
%GC47

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATA2520610.1398509514132447No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATAC2473830.13725545766089442No Hit
CCATGTACTCTGCGTTGATACCACTGCTTCCCACGTACTCTGCGTTGATA1835760.10185343332224266No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCATAC11390650.0140.292541
GCATACA11371450.0139.451932
ATAGCGA2608750.0136.005771
TAGCGAC2644700.0134.515092
GCTGATC3904900.0134.338932
AGGCTAA3401800.0133.957121
GTGCGAA3958450.0133.487932
GGCTAAC3432450.0133.389082
GGCTTCA7063550.0133.27662
ATCATTC5516700.0132.403291
CAGATTC6938050.0132.15822
CATACAG3931150.0131.972243
AGATTCG2303650.0131.09423
CCTAATC4332450.0130.750061
ACAGATT7013050.0130.621341
CTAATCC4318900.0130.37182
CATACAT4503700.0129.827213
TGGCATA2715400.0129.519152
ATGCCTA1364500.0128.356931
ACGCTTA1527600.0127.250012
Produced by FastQC (version 0.11.5)