FastQCFastQC Report
Sun 19 Mar 2023
SRR6026840_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6026840_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences180235456
Sequences flagged as poor quality0
Sequence length150
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAG50605182.8077261335305743No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGCAACCCATCTCTCCATCACG16928630.9392508208817694No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAGGCATTGAGGCAGCCAGCG16457820.9131288795918158No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGA12693340.7042643152299622No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGATCAAGTGAATAAGTGCA9532800.5289081411373354No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGAGGCATTGAGGCAGCCAGC9144370.5073568876481218No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGAATTCTGGACATTAATTA8670010.48103798178311824No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAATCTGGTTGATCCTGCCAG7576530.4203684540293781No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGGAATTCTGGACATTAATT6667160.3699138975185881No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGACTCTTCTGGTCCCCACAGA5451390.30245935627671394No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGCCAAAAAGAAAGGAGTTCAA4753390.26373223701334325No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGTCAAGCTACAAAGGGCTAAT3581050.1986873215445467No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGTGTGGGTGTTTGTGGTCAAG3394370.1883297590458561No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGACACTCGCTTCTGGAACGTC2750850.15262535247226827No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGGAATTCTCTTGCTTCAAC2402750.13331172752158155No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGATCAAGTGAATAAGTGCAT2357840.13081998693975064No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGCGTGTTTTTCGTGGTAATGG2315990.12849802427331503No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCAGT120723900.0116.567051
GTATCAA123846300.0113.588669
AGCAGTG123947700.0113.4963762
GGTATCA123921750.0113.4831168
GTGGTAT123946300.0113.440486
CAGTGGT124138700.0113.315284
AGTGGTA124286650.0113.157525
TGGTATC124275650.0113.1510247
GCAGTGG126232050.0111.41933
CATGGGG33553350.026.41716225-29
ATGGGGG13918450.025.3741725-29
CATGGGC16055200.024.8659725-29
CGATTAC1564000.024.63270465-69
ATGGGAC7252700.024.4949325-29
CGCAGAT3448150.024.35780590-94
ATGGGGT6397050.024.3533725-29
GCGATTA1613000.024.04865665-69
TCACGAT2478650.023.87908445-49
ACATGGG116415250.023.86358820-24
ATGGGAG14383000.023.57894525-29
Produced by FastQC (version 0.11.5)