FastQCFastQC Report
Sun 19 Mar 2023
SRR6026839_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6026839_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences228698586
Sequences flagged as poor quality0
Sequence length150
%GC50

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATAC24347191.0645973123769117No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT8517630.37243911949678604No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCCGCGTTGATAC8260670.36120337009866776No Hit
ACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACT7856490.34353032685562823No Hit
ACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCACGTACT5971340.26110087099532836No Hit
AACAACCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGT4792420.20955179845318328No Hit
CATGTACTCTGCGTTGATACCACTGCTTCGCATGTACTCTGCGTTGATAC3978400.1739582246477029No Hit
CCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATA2979300.13027190294915073No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGGGAC17949350.0140.113045
GAGTAGG35209050.0138.474722
TAGGGCC11078550.0138.17795
AGTAGGG29363900.0137.537843
AGGGCCT12530350.0135.62546
AGAGTAG41336700.0134.791061
GATAGAC6328400.0134.266081
GGGACTG8667100.0131.891337
TAGTGAC3448450.0131.558075
GAGTAGT6863400.0130.558522
ATAGACA6236200.0130.332952
ATGCCTA2652850.0128.917621
TGCCTAA2523450.0126.241292
AGTGACT3647550.0126.2403266
AGTAGTG5754500.0125.3916853
TCACGCA3452200.0125.3491742
GGGCCTC8312600.0124.897377
AGGGACT22198750.0122.6717156
GTAGGGC12275650.0122.298244
TAGACAT2406200.0122.247253
Produced by FastQC (version 0.11.5)