FastQCFastQC Report
Sun 19 Mar 2023
SRR6026837_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6026837_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences222614606
Sequences flagged as poor quality0
Sequence length150
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATAC6262620.2813211636257147No Hit
AGATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATA3603870.16188829945866176No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT3455720.15523330037023716No Hit
CCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATA3383660.1519963160009366No Hit
CCATGTACTCTGCGTTGATACCACTGCTTCCCATGGACTCTGCGTTGATA2874430.12912135693378538No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATGCCTA11643000.0143.198521
GCGAGTA26679850.0142.813541
CGAGTAA25910300.0142.386492
TGCCTAA11291450.0142.070622
AACGTGA9095700.0141.766451
ACGTGAT9080800.0141.021132
GCTGATC4877500.0140.354722
ACGTATC2830450.0140.239671
GCCTAAT3630150.0139.598363
GCCTAAG4602500.0139.472663
CGAACTT6241200.0139.347471
AGAGTCA3084300.0139.252791
GAACTTA6055000.0139.070432
CGCTGAT4915200.0139.016631
CAAGCTA2177500.0138.14882
TATCAGC1956550.0138.095371
AGCACCT3350200.0137.943981
ATCAGCA1882050.0137.435242
CTGATCA1430600.0137.43363
GAGTCAA3011500.0137.067812
Produced by FastQC (version 0.11.5)