FastQCFastQC Report
Sun 19 Mar 2023
SRR6026836_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6026836_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences208764687
Sequences flagged as poor quality0
Sequence length150
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CACTGTG28150800.0137.28581
ACTGTGT24680550.0137.144822
CTGTGTC13914950.0133.908133
GCCACAT7577800.0133.038471
TACGCAA3806700.0132.207862
GTGCGAA5540350.0131.681922
GATAGAC3886900.0131.524861
TATCAGC3714450.0131.325671
AGTGGTC6985950.0130.996841
CTAACGA5230700.0130.976352
TAAGGTC3992800.0130.925452
CATTGGC5718650.0129.68692
GTACGCA4393900.0129.243791
GTGGTCA6319700.0128.436452
CATCAAG3783200.0128.2981
CTAAGGT4398400.0127.862961
GTGTCCC10327600.0127.722875
ATCAGCA3450750.0127.2798542
TGTGTCC9856200.0127.24044
GGCTAAC3231800.0126.65832
Produced by FastQC (version 0.11.5)