FastQCFastQC Report
Sun 19 Mar 2023
SRR6026835_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6026835_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences200739060
Sequences flagged as poor quality0
Sequence length150
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACCGTC5391200.0136.738053
GTTACCG9717650.0136.046751
ACGTATC4901600.0135.049031
TTACCGT8032950.0134.435152
GTCGTAG5266400.0134.166141
GCCACAT9446600.0133.913961
TCGTAGA4688650.0131.84262
CGCATAC6180100.0131.37311
CTAACGA3147500.0128.210532
GCTAACG3561800.0128.087451
CCGAAGT3760700.0127.503421
GCATACA5872600.0127.3482742
CGAACTT3491600.0126.5751651
ACTTCGA2115450.0124.270732
CCGTCTA2489500.0124.1657942
CGAAGTA3594900.0123.7798842
ACCGTCC4576600.0123.137934
GCTGATC4104550.0123.0909652
CCACATA9343650.0123.0794452
TATCAGC3194700.0122.148061
Produced by FastQC (version 0.11.5)