FastQCFastQC Report
Sun 19 Mar 2023
SRR6026835_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6026835_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences200739060
Sequences flagged as poor quality0
Sequence length150
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAG120667416.011157469801842No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGA21822571.0871112976218977No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAGGCATTGAGGCAGCCAGCG4593650.22883687908073297No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGTATCAACGCAGAGTACATGG3228680.1608396492441481No Hit
AGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGA2893080.1441214280867909No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGAGGCATTGAGGCAGCCAGC2339830.11656077297562317No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGTGGTATCAACGCAGAGTACA2143010.1067560045364365No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCAGT148935300.075.314821
AGTGGTA151416200.074.1799245
AGCAGTG151533000.074.0760962
CAGTGGT151665100.074.0293664
GTATCAA152563000.073.695159
TGGTATC152252100.073.671677
GTGGTAT152805750.073.480596
GGTATCA153031700.073.3658
GCAGTGG154993250.072.4016953
ATGGGAT13290600.023.8297325-29
CATGGGG16065350.022.5331825-29
ATGGGAC6378750.022.09819425-29
CATGGGC10535750.021.57985525-29
ATGGGCG1578500.021.25047325-29
ATGGGGG4964250.020.81859425-29
ATGGGCT3944550.019.99916625-29
ATGGGAG17033350.019.59516725-29
TGGGATA3783900.019.44294725-29
ATGGGGC3092850.018.89929225-29
ATGGGGT4862500.018.64390825-29
Produced by FastQC (version 0.11.5)